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KMID : 0359319930330020255
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Korean Journal of Veterinary Research
1993 Volume.33 No. 2 p.255 ~ p.261
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Immunohistochemical diagnosis on rabies virus using its monoclonal antibody in mice
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Abstract
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For a immunohistochemical diagnosis of the frozen and paraffin-embedded tissues against rabies virus, mice were intracerebrally inoculated with challege virus standard (CVS) rabies virus and tlhen were used to detect the rabies viral antigen by the immunoperoxidase (IP) and the avidin-biotin complex(ABC) method.
In this study, the results confirmed that ABC and IP methods, although the former showed more specific and sensitive than the latter, were reliable and effective for the demonstration of rabies virus in both frozen and paraffin-embedded brain tissues prepared from rabies-infected mice. Additionally, IP technique using the monoclonal antibody against rabies virus could be recommended as a standard diagnostic tool instead of the present immunofluorescent method for the local veterinary services in Korea.
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KEYWORD
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